ORC ID , Jie Yu2, Yi-Fan Wang3, Qian-Qian Shan4 ORC ID , Ya-Xian Wang MD 1 ORC ID ">
  • Users Online: 765
  • Home
  • Print this page
  • Email this page
RESEARCH ARTICLE
Year : 2022  |  Volume : 17  |  Issue : 6  |  Page : 1387-1392

Selection of suitable internal controls for gene expression normalization in rats with spinal cord injury


1 Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, National Medical Products Administration (NMPA) Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, Jiangsu Province, China
2 Department of Nursing, The Affiliated Hospital of Nantong University; Department of Clinical Medicine, Medical School of Nantong University, Nantong, Jiangsu Province, China
3 Department of Clinical Medicine, Medical School of Nantong University, Nantong, Jiangsu Province, China
4 Department of Radiotherapy and Oncology, The Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China

Correspondence Address:
Qian-Qian Shan
Department of Radiotherapy and Oncology, The Affiliated Hospital of Nantong University, Nantong, Jiangsu Province
China
Ya-Xian Wang
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, National Medical Products Administration (NMPA) Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Nantong University, Nantong, Jiangsu Province
China
Login to access the Email id

Source of Support: This study was supported by the National Natural Science Foundation of China, No. 81901257 (to YXW); the Natural Science Foundation of Jiangsu Province of China, No. BK20180951 (to YXW); Postgraduate Research and Practice Innovation Program of Jiangsu Province of China, No. KYCX20_2818 (to WL); and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, to Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education), Conflict of Interest: None


DOI: 10.4103/1673-5374.327350

Rights and Permissions

There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research. In this study, we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes: Actb, Ankrd27, CypA, Gapdh, Hprt1, Mrpl10, Pgk1, Rictor, Rn18s, Tbp, Ubc, Ubxn11, and Ywhaz. Our results show that the expression of Ankrd27, Ubc, and Tbp were stable after spinal cord injury, while Actb was the most unstable internal control gene. Ankrd27, Ubc, Tbp, and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression. Target gene expression levels and changes over time were similar when Ankrd27, Ubc, and Tbp were used as internal controls but different when Actb was used as an internal control. We recommend that Ankrd27, Ubc, and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research. This study was approved by the Administration Committee of Experimental Animals, Jiangsu Province, China (approval No. 20180304-008) on March 4, 2018.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed270    
    Printed0    
    Emailed0    
    PDF Downloaded27    
    Comments [Add]    

Recommend this journal