|Year : 2022 | Volume
| Issue : 8 | Page : 1761-1768
Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies
, Yan-Di Yang1, Xi-Min Hu2, Wen-Ya Ning3, Lyu-Shuang Liao1, Shuang Lu1, Wen-Juan Zhao1, Qi Zhang MD 1
, Kun Xiong MD 4
1 Department of Neurobiology and Human Anatomy, School of Basic Medical Science, Central South University, Changsha, Hunan Province, China
2 Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China
3 Department of Human Resources, Third Xiangya Hospital of Central South University, Changsha, Hunan Province, China
4 Department of Neurobiology and Human Anatomy, School of Basic Medical Science, Central South University; Hunan Key Laboratory of Ophthalmology, Changsha, Hunan Province, China
|Date of Submission||03-Aug-2021|
|Date of Decision||15-Sep-2021|
|Date of Acceptance||01-Nov-2021|
|Date of Web Publication||07-Jan-2022|
Department of Neurobiology and Human Anatomy, School of Basic Medical Science, Central South University; Hunan Key Laboratory of Ophthalmology, Changsha, Hunan Province
Department of Neurobiology and Human Anatomy, School of Basic Medical Science, Central South University, Changsha, Hunan Province
Source of Support: The study was supported by the National Natural Science Foundation of China, Nos. 81772134 (to KX), 81971891 (to KX), 82172196 (to KX), 81571939 (to KX); the Fundamental Research Funds for the Central Universities of Central South University of China, No. 2020zzts218, (to WTY); Hunan Provincial Innovation Foundation For Postgraduate of China, Nos. CX20200116 (to WTY), CX20190139 (to LSL), Conflict of Interest: None
Some scholars have recently developed the concept of PANoptosis in the study of infectious diseases where pyroptosis, apoptosis and necroptosis act in consort in a multimeric protein complex, PANoptosome. This allows all the components of PANoptosis to be regulated simultaneously. PANoptosis provides a new way to study the regulation of cell death, in that different types of cell death may be regulated at the same time. To test whether PANoptosis exists in diseases other than infectious diseases, we chose cerebral ischemia/reperfusion injury as the research model, collected articles researching cerebral ischemia/reperfusion from three major databases, obtained the original research data from these articles by bibliometrics, data mining and other methods, then integrated and analyzed these data. We selected papers that investigated at least two of the components of PANoptosis to check its occurrence in ischemia/reperfusion. In the cell model simulating ischemic brain injury, pyroptosis, apoptosis and necroptosis occur together and this phenomenon exists widely in different passage cell lines or primary neurons. Pyroptosis, apoptosis and necroptosis also occurred in rat and mouse models of ischemia/reperfusion injury. This confirms that PANoptosis is observed in ischemic brain injury and indicates that PANoptosis can be a target in the regulation of various central nervous system diseases.
Keywords: apoptosis; brain; central nervous system; ischemia/reperfusion; middle cerebral artery occlusion; necroptosis; oxygen and glucose deprivation; PANoptosis; pyroptosis; regulated cell death
|How to cite this article:|
Yan WT, Yang YD, Hu XM, Ning WY, Liao LS, Lu S, Zhao WJ, Zhang Q, Xiong K. Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies. Neural Regen Res 2022;17:1761-8
|How to cite this URL:|
Yan WT, Yang YD, Hu XM, Ning WY, Liao LS, Lu S, Zhao WJ, Zhang Q, Xiong K. Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies. Neural Regen Res [serial online] 2022 [cited 2022 Jan 18];17:1761-8. Available from: http://www.nrronline.org/text.asp?2022/17/8/1761/331539
| Introduction|| |
Researchers studying forms of cell death found that the main processes of regulated cell death (RCD) included pyroptosis, apoptosis and regulated necrosis (including necroptosis) (Chen et al., 2021; Hu et al., 2021; Yan et al., 2021). The majority of the research topics on RCD focused on one of these three forms of cell death alone, but a few focused on the simultaneous interaction of these three forms of cell death. Some previous reports into cancer or bacterial/viral infection found that the key regulatory proteins of pyroptosis, apoptosis and necroptosis interacted with each other (Malireddi et al., 2010; Gurung et al., 2014, 2016; Malireddi et al., 2018, 2020b; Jiang et al., 2021; Meng et al., 2021). However, it was not clear how the regulatory mechanisms of pyroptosis, apoptosis and necroptosis intersected. The later research indicated that an innate immune response can simultaneously regulate pyroptosis, apoptosis and necroptosis after the transforming growth factor beta-activated kinase 1 (TAK1) was suppressed or knocked out (Malireddi et al., 2019, 2020b). This view was confirmed in research on coronavirus disease 2019 (COVID-19) (Karki et al., 2021). This suggests that, in the pathophysiological process of some diseases, pyroptosis, apoptosis and necroptosis can occur and be regulated at the same time. In a study by the team of Professor Kanneganti (Malireddi et al., 2019), this phenomenon when pyroptosis (P), apoptosis (A) and necroptosis (N) are regulated at the same time was named PANoptosis, and they showed that there is a multimeric protein complex, named a PANoptosome (Christgen et al., 2020; Samir et al., 2020), that can regulate the occurrence of PANoptosis.
A series of studies on PANoptosis reported by the Kanneganti team (Karki et al., 2020b, 2021; Kesavardhana et al., 2020; Malireddi et al., 2020a; Zheng et al., 2020; Briard et al., 2021) suggest that, in diseases caused by bacterial, fungal or viral infection, pathogens induce the autoimmune response and produce various inflammatory cytokines. These inflammatory cytokines activate the promoter proteins of pyroptosis, apoptosis and necroptosis through specific pathways, and drive them to assemble inflammasomes that are specific to different RCD forms (Cain et al., 2000; Chu et al., 2001; Acehan et al., 2002; Martinon et al., 2002; Agostini et al., 2004; Ogura et al., 2006; Kanneganti et al., 2007; Wallach et al., 2011; Lu et al., 2019b), and further assemble a protein complex, PANoptosome (Samir et al., 2020), that can simultaneously drive pyroptosis, apoptosis and necroptosis to aggravate cell death caused by the pathogens. Apart from diseases caused by pathogens, most other diseases or pathological conditions are more or less related to an immune response, which suggests that PANoptosis associated with an immune response is highly probable. For example, one study found that interferon regulatory factor 1, as the upstream regulator of PANoptosis, can induce cell death in the process of tumorigenesis in colorectal cancer (Karki et al., 2020a). In addition, in the exploration of the treatment of melanoma, a compound of metformin and doxorubicin initiated pyroptosis, apoptosis and necroptosis (PANoptosis) of melanoma cells, reducing the development of the melanoma (Song et al., 2021).
Published studies related to PANoptosis mainly focus on diseases induced by bacterial or viral infections plus a few types of tumors (Karki et al., 2020a, b; Malireddi et al., 2020b; Song et al., 2021). It is unknown whether PANoptosis and PANoptosomes exist in other types of diseases but it is worth further investigation. Many central nervous system (CNS) diseases involve the death of nerve cells, including PANoptosis (Yuan and Yankner, 2000; McKenzie et al., 2020; Yan et al., 2021). All these diseases or pathological conditions are generally associated with inflammatory reactions (Pender and Rist, 2001; Hoffmann et al., 2009; Degterev et al., 2019; Voet et al., 2019; Yuan et al., 2019; Lünemann et al., 2021). The expression of cell death and the pathophysiological mechanism related to inflammation in these CNS diseases are similar to the phenotype and mechanism in the existing studies of PANoptosis, which provides basic evidence for the possible existence of PANoptosis and PANoptosomes in CNS diseases.
In the Web of Science database, we investigated the experimental research articles about pyroptosis, apoptosis and necroptosis in the field of the nervous system and sorted the related articles according to the citation frequency, from high to low. Selecting the top 2% articles (referring to and expanding Essential Science Indicators standards) for keyword extraction and analysis, it was found that ischemia accounted for the highest proportion among the three death forms of PANoptosis in nervous system. Stroke is the second major cause of disability and death in adults, with ischemic stroke accounting for the majority of all stroke cases (Virani et al., 2020), and the main injury of ischemic stroke is caused by ischemia/reperfusion (I/R) (Meschia and Brott, 2018; Campbell et al., 2019; Yan et al., 2020a). The pathophysiological state of I/R can cause serious brain damage, and the pathophysiological process frequently involves an inflammatory reaction and immune system activation (Chamorro et al., 2016; Lambertsen et al., 2019; Shi et al., 2019; Yan et al., 2020b). Following the above argument we chose ischemia injury of the CNS as the analysis object.
We use bibliometrics, knowledge discovery and data-mining methods to capture evidence and analyze bibliometrics on the research of RCD related to ischemic injury of the CNS (Yan et al., 2020b) to assess the experimental research evidence on the involvement of PANoptosis in nervous system diseases. The demonstration of PANoptosis in ischemic injury of the CNS broadens the scope of PANoptosis research. This study takes a new approach to RCD research by exploring multiple RCD synchronously, pluralistically and comprehensively in ischemic injury of the CNS, and explores new ways to improve the intervention efficiency of RCD in nervous system diseases.
| Materials and Methods|| |
We chose PubMed, Scopus and Web of Science as the target databases. The key words were divided into three groups: (1) RCD, including pyroptosis, apoptosis and necroptosis; (2) CNS and their MeSH appositive words, hyponyms or hypernyms; and (3) ischemia. The refining function of the database limited the retrieval field to neuroscience or neurosurgery or neurology. The article type was limited to research articles. The retrieval of literature was completed on June 20, 2021. The end time of the publishing time range of the literature collections retrieved, with three cell death forms as the core theme, was June 20, 2021 but their start times differed as follows: (1) PubMed database: pyroptosis was on November 1, 2018; apoptosis was on May 1, 1995; necroptosis was on January 12, 2007. (2) Scopus database: The starting time of pyroptosis was on July 1, 2008; apoptosis on December 24, 1993. necroptosis started on July 1, 2005. (3) Web of Science database: The starting time of pyroptosis was on April 1, 2014; apoptosis on December 24, 1993; necroptosis on July 1, 2005. The retrieval strategy of each database was customized according to the usage standard of the database and the scale of the retrieved documents. Articles retrieved from each database were merged according to the three forms of cell death, and duplicate documents were screened and removed according to the inclusion criteria. The process of literature screening was shown in [Figure 1].
Studies were potentially included if they met the following criteria: (1) The core content of the paper was to study ischemia or I/R injury or animal or cell models that can represent ischemia or I/R; (2) Rodents or primary cells or subculture cell lines were used as the experimental materials; (3) The target organ damaged in the experiment was either the brain or primary cells and subculture cells that can represent neurons; (4) The experimental results included two or more corresponding detection results that proved the existence of the three kinds of cell death: pyroptosis, apoptosis and necroptosis, one of which must be the key protein detection results of these three kinds of cell death forms; and (5) Damage treatment group and blank control group were included in the experimental design.
Studies were excluded if they met any of the criteria: (1) Drug-induced animal model or cell model; (2) The target cells of the experimental study were non-neuronal cells (glial cells, endothelial cells, etc.); (3) The process and standard description of establishing the model were not given; and (4) The experimental evidence to prove the existence of any of the three cell death forms was insufficient.
Data mining and sorting analysis
Data such as cell types, animal species, modeling methods, evaluation of cell death and detection results of representative molecules of different cell death types were extracted from the included literature. The literature items exported from the database were imported into the literature management software, and two researchers with medical and biological knowledge independently read the literature one by one, conducted article selection and data mining, and obtained relevant data from the literature. The data obtained by the two researchers were compared, and the consistent results were summarized in a table. When any inconsistent results occurred, the discussion and decision for inclusion involved the participation of the third researcher. The cluster analysis of in vitro experiments was based on the cell type and had to be that used in the study of pyroptosis, apoptosis and necroptosis. Cluster analysis of in vivo experiments of animals was carried out according to the classification of common rodents, ensuring that the I/R operations performed on animals were of the same class. To summarize, the acquired core data was collated and analyzed using EndNote software (version X7.8, Clarivate Analytics, Boston, MA, USA) and Microsoft Excel software (version 2016, Microsoft Corporation, Redmond, WA, USA).
| Results|| |
A total of 57 articles were included in this study (18 articles in pyroptosis, 22 articles in apoptosis, and 17 articles in necroptosis; [Figure 1]), of which 22 were conducted on rodents only (including rats and mice) and 31 were conducted on primary cultured cells or cell lines only and 4 studies included both in vitro cell and in vivo rodent experiments. One of the 31 articles that had experimented on two types of cell. From the included literature, we extracted 62 experiments that assessed pyroptosis or apoptosis or necroptosis. Of these studies, it was necessary to satisfy two conditions that would determine whether I/R injury in the experiment induced the occurrence of pyroptosis or apoptosis or necroptosis. One condition was that commonly used or academically recognized detection methods were used in the experiment, such as propidium iodide staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) assay, flow cytometry, cell counting kit-8 assay or lactate dehydrogenase assay to evaluate the degree of cell death induced by I/R injury. The other condition was that the key proteins of pyroptosis or apoptosis or necroptosis were detected [Table 1] (Fink and Cookson, 2005; Bergsbaken et al., 2009; Kaczmarek et al., 2013; Nikoletopoulou et al., 2013; Czabotar et al., 2014; Kovacs and Miao, 2017; Hu et al., 2021) and that they should contain at least two or more key proteins. Both the conditions mentioned above had to give results that were statistically significant compared to the control group and be clearly stated in the paper.
In the 36 cell-model-based experiments, oxygen and glucose deprivation (OGD) or OGD/recovery was used in most cell experiments to simulate ischemia or I/R injury. The researchers used primary hippocampal cells, primary cortical cells, PC12 cells (rat adrenal pheochromocytoma cells) and SH-SY5Y (human neuroblastoma cells) cells for the experiments. We show the results according to the cell types used in the experiments [Table 2],[Table 3],[Table 4],[Table 5]. In the included studies, most were based on rodent models, middle cerebral artery occlusion (MCAO) or modified MCAO to simulate ischemia or I/R injury but some used the method of electric shock cardiac arrest and resuscitation. These modeling methods simulate cerebral I/R injury in experimental animals and are recognized in the research field. The studies used Sprague-Dawley rats or C57 mice, and we tabulated the results according to animal type and modeling method used in the experiment [Table 6] and [Table 7]. In the process of data mining, we found that experimental models, apart from MCAO and OGD models, did not meet the condition that pyroptosis, apoptosis and necroptosis were studied simultaneously. We extracted 62 experiments from the 57 included papers. According to the experimental results included in our analysis it appears that in the same cell model or animal disease model three kinds of RCD, i.e., pyroptosis, apoptosis, necroptosis, were likely to occur simultaneously, which would mean that PANoptosis occurs in these experiments.
|Table 2: Oxygen-glucose deprivation/reperfusion (OGD/R) induces death of primary hippocampal cells|
Click here to view
|Table 3: Oxygen-glucose deprivation/reperfusion (OGD/R) induces death of primary cortical cells|
Click here to view
|Table 4: Oxygen-glucose deprivation/reperfusion (OGD/R) induces death of PC12 cells|
Click here to view
|Table 5: Oxygen-glucose deprivation/reperfusion (OGD/R) induces death of SH-SY5Y cells|
Click here to view
|Table 6: Brain injury induced by ischemia/reperfusion (I/R) injury in rats|
Click here to view
|Table 7: Brain injury induced by ischemia/reperfusion (I/R) injury in mice|
Click here to view
| Discussion|| |
In this study we selected MCAO and OGD as in vivo and in vitro experimental models, respectively, that can simulate I/R injury and its pathophysiology in the CNS. These two methods are the most widely used and generally recognized by researchers (Ryou and Mallet, 2018; Salvador et al., 2018). Many have studied RCD induced by I/R injury of CNS using MCAO and OGD (Yanamoto et al., 2003; Tuttolomondo et al., 2009; McBride and Zhang, 2017; Ryou and Mallet, 2018; Wang et al., 2018; Li et al., 2019; Zhang et al., 2019a), and these two methods have often been used to study the inflammatory reaction related to this kind of injury (Tuttolomondo et al., 2009; Rizzo and Leaver, 2010; Mo et al., 2020a; Stanzione et al., 2020; Huang et al., 2021). Therefore, it is pertinent to discuss PANoptosis in MCAO and OGD models.
Kanneganti’s proposal is that PANoptosis is a newly defined form of cell death in diseases related to the immune response and can be regulated by a multimeric protein complex, named PANoptosome (Malireddi et al., 2019). This new form of cell death includes pyroptosis, apoptosis and necroptosis. He proposes that a PANoptosome can interfere with pyroptosis, apoptosis and necroptosis, each of which have been studied independently by other investigators. The existing research on PANoptosis suggests cysteinyl aspartate-specific protease (CASP) 1 and CASP-11 that drive pyroptosis, CASP-8 that drives apoptosis and RIP3 that drives necroptosis can all be assembled into a PANoptosome, together with other components. The process of PANoptosis can be regulated by Z-DNA-binding protein 1 and TAK1 (Christgen et al., 2020; Samir et al., 2020). To support the theory that PANoptosis is a major factor in the I/R injury of the CNS first it is necessary to confirm that pyroptosis, apoptosis and necroptosis have been shown to occur simultaneously from reports in existing literature on I/R injury. Second, a PANoptosome has to have been identified in I/R injury, and have been confirmed that it can simultaneously initiate the three kinds of RCD. Third, there must be a regulatory system that controls PANoptosome activity.
The data we mined from the literature showed that in the study of cerebral I/R, under the same model condition, the three forms of cell death could occur simultaneously. According to our integrated data, after MCAO induced I/R injury in rat or mouse brain tissue and OGD induced ischemia-hypoxia injury in neurons or cell lines derived from nerve cells, pyroptosis, apoptosis and necroptosis coexisted. This phenomenon accords with the first condition of the PANoptosis definition, and suggests that it is very possible that PANoptosis exists in nervous system diseases from the phenomenon level or the phenotype level of cerebral ischemia injury. We can see from the related studies of the three kinds of RCD—pyroptosis, apoptosis and necroptosis—that the molecular mechanisms of these three kinds of cell death all have inflammation-related parts (Linkermann et al., 2013; Lu et al., 2019a; Guo et al., 2020; Wang et al., 2020c, 2021b; Chen et al., 2021; Liu et al., 2021c). There are also reports that glial cells can interfere with these three forms of cell death after being stimulated by injury (Zhao et al., 2017; Xu et al., 2019; Naito et al., 2020; Wang et al., 2020a; Li et al., 2021a; Liu et al., 2021b; Lu et al., 2021) and these overlap with the inflammation-related and immune-related reports of existing studies of PANoptosis. This suggests the possibility of PANoptosis in CNS diseases at the pathological mechanism level.
The latest research suggests that a PANoptosome includes three kinds of protein: (1) Z-DNA-binding protein 1, a nucleotide-binding domain and a leucine-rich repeat pyrin-domain containing protein 3 that play the role of sensor, (2) an apoptosis-associated speck-like protein, containing a caspase recruit domain, and a Fas-associated protein with death domain that are composite adapters and (3) a receptor-interacting protein kinase (RIP) 1, RIP3, CASP-1 and CASP-8 that have a catalytic effect (Christgen et al., 2020; Samir et al., 2020; Zheng and Kanneganti, 2020a, b). These studies on the PANoptosome are related to infectious diseases and cancer, but there has been no study on PANoptosomes in the study of I/R injury of CNS. It can be seen from the data mined by us that nucleotide-binding domain and leucine-rich repeat pyrin-domain containing protein 3, CASP-1 and apoptosis-associated speck-like protein containing a caspase recruit domain related to pyroptosis, CASP-8 and Fas-associated protein with death domain related to apoptosis, RIP1 and RIP3 related to necroptosis have all been detected as marker proteins in animal models of I/R and/or cell models of OGD/recovery [Table 2],[Table 3],[Table 4],[Table 5],[Table 6],[Table 7]. All these proteins are considered to be components of a PANoptosome in infectious diseases. Although there is no study on the assembly of components of a PANoptosome in I/R injury of CNS, the existing data of the “raw materials” that make up a PANoptosome are highly expressed, indicating that there is a molecular basis for finding PANoptosomes in ischemia-induced brain injury.
There are studies that showed there are some molecules that can interfere with two of the components of PANoptosis simultaneously under the condition of I/R injury. For example, nucleotide oligomerization domain-like receptors with caspase activation and recruitment domain 4 inflammasome complex can regulate apoptosis and pyroptosis (Poh et al., 2019). Also blocking thromboxane A synthase/thromboxane A2/thromboxane prostanoid signal can inhibit apoptosis and pyroptosis at the same time (Chueh et al., 2020). RIPK3, as the key molecule of necroptosis (Kikuchi et al., 2012; Sun et al., 2012; Kim and Li, 2013; Thapa et al., 2013; Guo et al., 2020; Wang et al., 2020b; Liao et al., 2021), can interact with the Jun N-terminal kinase-mediated inflammatory signaling pathway (Hu et al., 2020) that is closely related to neuronal apoptosis induced by ischemia (Wang et al., 2011; Liu et al., 2016, 2018) and to cell pyroptosis (Chen et al., 2019; Jiang et al., 2020a). All this information suggests that pyroptosis, apoptosis and necroptosis (PANoptosis) induced by I/R injury could be subject to intervention and regulation simultaneously.
The existing studies on PANoptosis show that TAK1 and Z-DNA-binding protein 1 intervene in PANoptosome activity, and thus participate in the regulation of PANoptosis (Malireddi et al., 2019; Banoth et al., 2020; Kesavardhana et al., 2020; Samir et al., 2020; Zheng and Kanneganti, 2020b). We have not found any internal or external molecules that can interfere with all three of pyroptosis, apoptosis and necroptosis in cerebral ischemia injury, but some studies have shown that inhibiting TAK1 can reduce neuronal death induced by cerebral I/R (Neubert et al., 2011). This indicates that TAK1 can be used as an important target in RCD induced by hypoxia-reperfusion injury (Neubert et al., 2011; Ridder and Schwaninger, 2013; Wang et al., 2019; Wu et al., 2020b). TAK1 can affect the function of microglia and interact with inflammatory pathway, thus affecting neuronal apoptosis and pyroptosis (Gong et al., 2015; Zeyen et al., 2020). It also plays an important role in the interaction between programmed necrosis and apoptosis of neurons mediated by RIP3 during cerebral I/R injury (Naito et al., 2020). All these data suggest that there may be molecules, like TAK1, that can regulate PANoptosomes in a brain subject to I/R injury.
Although this paper verifies the possibility of PANoptosis in cerebral ischemia reperfusion injury by collecting data from cell experiments and animal experiments, we admit that this paper has some limitations. First, the limits of paper length and research scale meant we could not conduct data mining for all CNS diseases, therefore we selected only cerebral I/R injury as the research object. This limited the outcome to only showing whether PANoptosis exists in cerebral I/R injury. Whether PANoptosis occurs in other CNS diseases remains to be studied. Second, the data we mined were mainly cell experiments and animal experiments, without clinical samples. Whether PANoptosis occurs in actual clinical stroke needs further verification. Third, the disease models we analyzed were only MCAO and OGD, therefore other ischemia/reperfusion models would need to be studied. Fourth, we only selected three databases for retrieval, whereas there are other databases. Fifth, our retrieval fields are mainly from title, abstract and keywords, so some relevant papers may have been missed. These limitations need to be addressed in future studies.
Summary and future directions
Analysis of existing research highlights how important PANoptosis is and shows how its interaction network of processes is associated with RCD in infectious diseases. The concept of PANoptosis improves our understanding of RCD, suggesting that we should treat and understand RCD systematically, plurally and as a network. Although the current research focuses mainly on infectious diseases, this review proposes expanding investigations of PANoptosis to other diseases. In the pathophysiological mechanism of CNS diseases the inflammatory response and immune response play important roles that are similar to their effects in infectious diseases. Moreover, there are interactions between regulatory proteins that regulate the disease response and immune response of CNS diseases. However, systematic and comprehensive research on these interactions still needs further study. In future, the research on PANoptosis in CNS diseases should examine the interaction network of key regulatory proteins, identify a PANoptosome linked to CNS diseases, find the target of PANoptosis that can intervene in neurons and find new treatment strategies for diseases related to RCD.
Author contributions: All authors contributed equally to the manuscript, read and approved the final version of the paper for publication.
Conflicts of interest: The authors declare that there is no potential conflict of interest.
©Article author(s) (unless otherwise stated in the text of the article) 2022. All rights reserved. No commercial use is permitted unless otherwise expressly granted.
Funding: The study was supported by the National Natural Science Foundation of China, Nos. 81772134 (to KX), 81971891 (to KX), 82172196 (to KX), 81571939 (to KX); the Fundamental Research Funds for the Central Universities of Central South University of China, No. 2020zzts218, (to WTY); Hunan Provincial Innovation Foundation For Postgraduate of China, Nos. CX20200116 (to WTY), CX20190139 (to LSL).
| References|| |
Acehan D, Jiang X, Morgan DG, Heuser JE, Wang X, Akey CW (2002) Three-dimensional structure of the apoptosome: implications for assembly, procaspase-9 binding, and activation. Mol Cell 9:423-432.
Agostini L, Martinon F, Burns K, McDermott MF, Hawkins PN, Tschopp J (2004) NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder. Immunity 20:319-325.
An P, Xie J, Qiu S, Liu Y, Wang J, Xiu X, Li L, Tang M (2019) Hispidulin exhibits neuroprotective activities against cerebral ischemia reperfusion injury through suppressing NLRP3-mediated pyroptosis. Life Sci 232:116599.
Asadi Y, Gorjipour F, Behrouzifar S, Vakili A (2018) Irisin peptide protects brain against ischemic injury through reducing apoptosis and enhancing BDNF in a rodent model of stroke. Neurochem Res 43:1549-1560.
Banoth B, Tuladhar S, Karki R, Sharma BR, Briard B, Kesavardhana S, Burton A, Kanneganti TD (2020) ZBP1 promotes fungi-induced inflammasome activation and pyroptosis, apoptosis, and necroptosis (PANoptosis). J Biol Chem 295:18276-18283.
Bergsbaken T, Fink SL, Cookson BT (2009) Pyroptosis: host cell death and inflammation. Nat Rev Microbiol 7:99-109.
Briard B, Malireddi RKS, Kanneganti TD (2021) Role of inflammasomes/pyroptosis and PANoptosis during fungal infection. PLoS Pathog 17:e1009358.
Cai HA, Tao X, Zheng LJ, Huang L, Peng Y, Liao RY, Zhu YM (2020) Ozone alleviates ischemia/reperfusion injury by inhibiting mitochondrion-mediated apoptosis pathway in SH-SY5Y cells. Cell Biol Int 44:975-984.
Cain K, Bratton SB, Langlais C, Walker G, Brown DG, Sun XM, Cohen GM (2000) Apaf-1 oligomerizes into biologically active approximately 700-kDa and inactive approximately 1.4-MDa apoptosome complexes. J Biol Chem 275:6067-6070.
Campbell BCV, De Silva DA, Macleod MR, Coutts SB, Schwamm LH, Davis SM, Donnan GA (2019) Ischaemic stroke. Nat Rev Dis Primers 5:70.
Cao G, Zhou H, Jiang N, Han Y, Hu Y, Zhang Y, Qi J, Kou J, Yu B (2016) YiQiFuMai powder injection ameliorates cerebral ischemia by inhibiting endoplasmic reticulum stress-mediated neuronal apoptosis. Oxid Med Cell Longev 2016:5493279.
Chamorro Á, Dirnagl U, Urra X, Planas AM (2016) Neuroprotection in acute stroke: targeting excitotoxicity, oxidative and nitrosative stress, and inflammation. Lancet Neurol 15:869-881.
Chang CF, Lai JH, Wu JC, Greig NH, Becker RE, Luo Y, Chen YH, Kang SJ, Chiang YH, Chen KY (2017) (-)-Phenserine inhibits neuronal apoptosis following ischemia/reperfusion injury. Brain Res 1677:118-128.
Chen S, Zuo Y, Huang L, Sherchan P, Zhang J, Yu Z, Peng J, Zhang J, Zhao L, Doycheva D, Liu F, Zhang JH, Xia Y, Tang J (2019) The MC(4) receptor agonist RO27-3225 inhibits NLRP1-dependent neuronal pyroptosis via the ASK1/JNK/p38 MAPK pathway in a mouse model of intracerebral haemorrhage. Br J Pharmacol 176:1341-1356.
Chen X, Zhang X, Xue L, Hao C, Liao W, Wan Q (2017) Treatment with enriched environment reduces neuronal apoptosis in the periinfarct cortex after cerebral ischemia/reperfusion injury. Cell Physiol Biochem 41:1445-1456.
Chen Y, Zhang L, Yu H, Song K, Shi J, Chen L, Cheng J (2018) Necrostatin-1 improves long-term functional recovery through protecting oligodendrocyte precursor cells after transient focal cerebral ischemia in mice. Neuroscience 371:229-241.
Chen Y, Li Y, Guo L, Hong J, Zhao W, Hu X, Chang C, Liu W, Xiong K (2021) Bibliometric analysis of the inflammasome and pyroptosis in brain. Front Pharmacol 11:626502.
Christgen S, Zheng M, Kesavardhana S, Karki R, Malireddi RKS, Banoth B, Place DE, Briard B, Sharma BR, Tuladhar S, Samir P, Burton A, Kanneganti TD (2020) Identification of the PANoptosome: a molecular platform triggering pyroptosis, apoptosis, and necroptosis (PANoptosis). Front Cell Infect Microbiol 10:237.
Chu ZL, Pio F, Xie Z, Welsh K, Krajewska M, Krajewski S, Godzik A, Reed JC (2001) A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis. J Biol Chem 276:9239-9245.
Chueh TH, Cheng YH, Chen KH, Chien CT (2020) Thromboxane A2 synthase and thromboxane receptor deletion reduces ischaemia/reperfusion-evoked inflammation, apoptosis, autophagy and pyroptosis. Thromb Haemost 120:329-343.
Czabotar PE, Lessene G, Strasser A, Adams JM (2014) Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy. Nat Rev Mol Cell Biol 15:49-63.
Degterev A, Ofengeim D, Yuan J (2019) Targeting RIPK1 for the treatment of human diseases. Proc Natl Acad Sci U S A 116:9714-9722.
Deng XX, Li SS, Sun FY (2019) Necrostatin-1 prevents necroptosis in brains after ischemic stroke via inhibition of RIPK1-mediated RIPK3/MLKL signaling. Aging Dis 10:807-817.
Diao MY, Zhu Y, Yang J, Xi SS, Wen X, Gu Q, Hu W (2020) Hypothermia protects neurons against ischemia/reperfusion-induced pyroptosis via m6A-mediated activation of PTEN and the PI3K/Akt/GSK-3β signaling pathway. Brain Res Bull 159:25-31.
Fink SL, Cookson BT (2005) Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells. Infect Immun 73:1907-1916.
Gao Y, Chen T, Lei X, Li Y, Dai X, Cao Y, Ding Q, Lei X, Li T, Lin X (2016) Neuroprotective effects of polydatin against mitochondrial-dependent apoptosis in the rat cerebral cortex following ischemia/reperfusion injury. Mol Med Rep 14:5481-5488.
Gong J, Li ZZ, Guo S, Zhang XJ, Zhang P, Zhao GN, Gao L, Zhang Y, Zheng A, Zhang XF, Xiang M, Li H (2015) Neuron-specific tumor necrosis factor receptor-associated factor 3 is a central regulator of neuronal death in acute ischemic stroke. Hypertension 66:604-616.
Guo LM, Wang Z, Li SP, Wang M, Yan WT, Liu FX, Wang CD, Zhang XD, Chen D, Yan J, Xiong K (2020) RIP3/MLKL-mediated neuronal necroptosis induced by methamphetamine at 39°C. Neural Regen Res 15:865-874.
Gurung P, Burton A, Kanneganti TD (2016) NLRP3 inflammasome plays a redundant role with caspase 8 to promote IL-1β-mediated osteomyelitis. Proc Natl Acad Sci U S A 113:4452-4457.
Gurung P, Anand PK, Malireddi RK, Vande Walle L, Van Opdenbosch N, Dillon CP, Weinlich R, Green DR, Lamkanfi M, Kanneganti TD (2014) FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes. J Immunol 192:1835-1846.
He G, Xu W, Tong L, Li S, Su S, Tan X, Li C (2016) Gadd45b prevents autophagy and apoptosis against rat cerebral neuron oxygen-glucose deprivation/reperfusion injury. Apoptosis 21:390-403.
He GQ, Xu WM, Liao HJ, Jiang C, Li CQ, Zhang W (2019) Silencing Huwe1 reduces apoptosis of cortical neurons exposed to oxygen-glucose deprivation and reperfusion. Neural Regen Res 14:1977-1985.
Hoffmann O, Zipp F, Weber JR (2009) Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) in central nervous system inflammation. J Mol Med (Berl) 87:753-763.
Hu W, Wu X, Yu D, Zhao L, Zhu X, Li X, Huang T, Chu Z, Xu Y (2020) Regulation of JNK signaling pathway and RIPK3/AIF in necroptosis-mediated global cerebral ischemia/reperfusion injury in rats. Exp Neurol 331:113374.
Hu XM, Li ZX, Lin RH, Shan JQ, Yu QW, Wang RX, Liao LS, Yan WT, Wang Z, Shang L, Huang Y, Zhang Q, Xiong K (2021) Guidelines for regulated cell death assays: a systematic summary, a categorical comparison, a prospective. Front Cell Dev Biol 9:634690.
Huang T, Gao D, Jiang X, Hu S, Zhang L, Fei Z (2014) Resveratrol inhibits oxygen-glucose deprivation-induced MMP-3 expression and cell apoptosis in primary cortical cells via the NF-κB pathway. Mol Med Rep 10:1065-1071.
Huang Y, Wang S, Huang F, Zhang Q, Qin B, Liao L, Wang M, Wan H, Yan W, Chen D, Liu F, Jiang B, Ji D, Xia X, Huang J, Xiong K (2021) c-FLIP regulates pyroptosis in retinal neurons following oxygen-glucose deprivation/recovery via a GSDMD-mediated pathway. Ann Anat 235:151672.
Jiang C, Shi R, Chen B, Yan X, Tang G (2020a) Casticin elicits inflammasome-induced pyroptosis through activating PKR/JNK/NF-κB signal in 5-8F cells. Biomed Pharmacother 123:109576.
Jiang M, Qi L, Li L, Wu Y, Song D, Li Y (2021) Caspase-8: A key protein of cross-talk signal way in “PANoptosis” in cancer. Int J Cancer 149:1408-1420.
Jiang Q, Geng X, Warren J, Eugene Paul Cosky E, Kaura S, Stone C, Li F, Ding Y (2020b) Hypoxia inducible factor-1α (HIF-1α) mediates NLRP3 inflammasome-dependent-pyroptotic and apoptotic cell death following ischemic stroke. Neuroscience 448:126-139.
Kaczmarek A, Vandenabeele P, Krysko DV (2013) Necroptosis: the release of damage-associated molecular patterns and its physiological relevance. Immunity 38:209-223.
Kanneganti TD, Lamkanfi M, Kim YG, Chen G, Park JH, Franchi L, Vandenabeele P, Núñez G (2007) Pannexin-1-mediated recognition of bacterial molecules activates the cryopyrin inflammasome independent of Toll-like receptor signaling. Immunity 26:433-443.
Karki R, Sharma BR, Lee E, Banoth B, Malireddi RKS, Samir P, Tuladhar S, Mummareddy H, Burton AR, Vogel P, Kanneganti TD (2020a) Interferon regulatory factor 1 regulates PANoptosis to prevent colorectal cancer. JCI Insight 5:e136720.
Karki R, Sharma BR, Tuladhar S, Williams EP, Zalduondo L, Samir P, Zheng M, Sundaram B, Banoth B, Malireddi RKS, Schreiner P, Neale G, Vogel P, Webby R, Jonsson CB, Kanneganti TD (2020b) Synergism of TNF-α and IFN-γ triggers inflammatory cell death, tissue damage, and mortality in SARS-CoV-2 infection and cytokine shock syndromes. bioRxiv doi: 10.1101/2020.10.29.361048.
Karki R, Sharma BR, Tuladhar S, Williams EP, Zalduondo L, Samir P, Zheng M, Sundaram B, Banoth B, Malireddi RKS, Schreiner P, Neale G, Vogel P, Webby R, Jonsson CB, Kanneganti TD (2021) Synergism of TNF-α and IFN-γ triggers inflammatory cell death, tissue damage, and mortality in SARS-CoV-2 infection and cytokine shock syndromes. Cell 184:149-168.e17.
Kesavardhana S, Malireddi RKS, Burton AR, Porter SN, Vogel P, Pruett-Miller SM, Kanneganti TD (2020) The Zα2 domain of ZBP1 is a molecular switch regulating influenza-induced PANoptosis and perinatal lethality during development. J Biol Chem 295:8325-8330.
Kikuchi M, Kuroki S, Kayama M, Sakaguchi S, Lee KK, Yonehara S (2012) Protease activity of procaspase-8 is essential for cell survival by inhibiting both apoptotic and nonapoptotic cell death dependent on receptor-interacting protein kinase 1 (RIP1) and RIP3. J Biol Chem 287:41165-41173.
Kim SJ, Li J (2013) Caspase blockade induces RIP3-mediated programmed necrosis in Toll-like receptor-activated microglia. Cell Death Dis 4:e716.
Kong D, Zhu J, Liu Q, Jiang Y, Xu L, Luo N, Zhao Z, Zhai Q, Zhang H, Zhu M, Liu X (2017) Mesenchymal stem cells protect neurons against hypoxic-ischemic injury via inhibiting parthanatos, necroptosis, and apoptosis, but not autophagy. Cell Mol Neurobiol 37:303-313.
Kovacs SB, Miao EA (2017) Gasdermins: effectors of pyroptosis. Trends Cell Biol 27:673-684.
Lambertsen KL, Finsen B, Clausen BH (2019) Post-stroke inflammation-target or tool for therapy? Acta Neuropathol 137:693-714.
Li C, Sui C, Wang W, Yan J, Deng N, Du X, Cheng F, Ma X, Wang X, Wang Q (2021a) Baicalin attenuates oxygen-glucose deprivation/reoxygenation-induced injury by modulating the BDNF-TrkB/PI3K/Akt and MAPK/Erk1/2 signaling axes in neuron-astrocyte cocultures. Front Pharmacol 12:599543.
Li F, Xu D, Hou K, Gou X, Lv N, Fang W, Li Y (2021b) Pretreatment of indobufen and aspirin and their combinations with clopidogrel or ticagrelor alleviates inflammasome mediated pyroptosis via inhibiting NF-κB/NLRP3 pathway in ischemic stroke. J Neuroimmune Pharmacol doi: 10.1007/s11481-020-09978-9.
Li HN, Zheng RF, Du YW, Wang W, Sun FL, Liu DW, Xing JG (2021c) Effect and mechanism of tilianin against necroptosis on cerebral ischemia-reperfusion. Zhongcaoyao 52:1974-1980.
Li J, Zhang J, Zhang Y, Wang Z, Song Y, Wei S, He M, You S, Jia J, Cheng J (2019) TRAF2 protects against cerebral ischemia-induced brain injury by suppressing necroptosis. Cell Death Dis 10:328.
Li J, Hao JH, Yao D, Li R, Li XF, Yu ZY, Luo X, Liu XH, Wang MH, Wang W (2020a) Caspase-1 inhibition prevents neuronal death by targeting the canonical inflammasome pathway of pyroptosis in a murine model of cerebral ischemia. CNS Neurosci Ther 26:925-939.
Li W, Liu J, Chen JR, Zhu YM, Gao X, Ni Y, Lin B, Li H, Qiao SG, Wang C, Zhang HL, Ao GZ (2018) Neuroprotective effects of DTIO, a novel analog of Nec-1, in acute and chronic stages after ischemic stroke. Neuroscience 390:12-29.
Li X, Cheng S, Hu H, Zhang X, Xu J, Wang R, Zhang P (2020b) Progranulin protects against cerebral ischemia-reperfusion (I/R) injury by inhibiting necroptosis and oxidative stress. Biochem Biophys Res Commun 521:569-576.
Liang J, Wang Q, Li JQ, Guo T, Yu D (2020a) Long non-coding RNA MEG3 promotes cerebral ischemia-reperfusion injury through increasing pyroptosis by targeting miR-485/AIM2 axis. Exp Neurol 325:113139.
Liang Y, Song P, Chen W, Xie X, Luo R, Su J, Zhu Y, Xu J, Liu R, Zhu P, Zhang Y, Huang M (2020b) Inhibition of caspase-1 ameliorates ischemia-associated blood-brain barrier dysfunction and integrity by suppressing pyroptosis activation. Front Cell Neurosci 14:540669.
Liao LS, Lu S, Yan WT, Wang SC, Guo LM, Yang YD, Huang K, Hu XM, Zhang Q, Yan J, Xiong K (2021) The role of HSP90α in methamphetamine/hyperthermia-induced necroptosis in rat striatal neurons. Front Pharmacol 12:716394.
Lin Y, Cai B, Xue XH, Fang L, Wu ZY, Wang N (2015) TAT-mediated delivery of neuroglobin attenuates apoptosis induced by oxygen-glucose deprivation via the Jak2/Stat3 pathway in vitro. Neurol Res 37:531-538.
Linkermann A, Hackl MJ, Kunzendorf U, Walczak H, Krautwald S, Jevnikar AM (2013) Necroptosis in immunity and ischemia-reperfusion injury. Am J Transplant 13:2797-2804.
Liu H, Zhao Z, Wu T, Zhang Q, Lu F, Gu J, Jiang T, Xue J (2021a) Inhibition of autophagy-dependent pyroptosis attenuates cerebral ischaemia/reperfusion injury. J Cell Mol Med 25:5060-5069.
Liu J, Wang Q, Yang S, Huang J, Feng X, Peng J, Lin Z, Liu W, Tao J, Chen L (2018) Electroacupuncture inhibits apoptosis of peri-ischemic regions via modulating p38, extracellular signal-regulated kinase (ERK1/2), and c-Jun N terminal kinases (JNK) in cerebral ischemia-reperfusion-injured rats. Med Sci Monit 24:4395-4404.
Liu X, Lv X, Liu Z, Zhang M, Leng Y (2021b) MircoRNA-29a in astrocyte-derived extracellular vesicles suppresses brain ischemia reperfusion injury via TP53INP1 and the NF-κB/NLRP3 axis. Cell Mol Neurobiol doi: 10.1007/s10571-021-01040-3.
Liu X, Zhang M, Liu H, Zhu R, He H, Zhou Y, Zhang Y, Li C, Liang D, Zeng Q, Huang G (2021c) Bone marrow mesenchymal stem cell-derived exosomes attenuate cerebral ischemia-reperfusion injury-induced neuroinflammation and pyroptosis by modulating microglia M1/M2 phenotypes. Exp Neurol 341:113700.
Liu Y, Jiang S, Yang PY, Zhang YF, Li TJ, Rui YC (2016) EF1A1/HSC70 cooperatively suppress brain endothelial cell apoptosis via regulating JNK activity. CNS Neurosci Ther 22:836-844.
Lu S, Yang Y, Liao L, Yan W, Xiong K, Yan J (2021) iTRAQ-based proteomic analysis of the rat striatum in response to methamphetamine preconditioning. Acta Biochim Biophys Sin (Shanghai) 53:636-639.
Lu S, Liao L, Zhang B, Yan W, Chen L, Yan H, Guo L, Lu S, Xiong K, Yan J (2019a) Antioxidant cascades confer neuroprotection in ethanol, morphine, and methamphetamine preconditioning. Neurochem Int 131:104540.
Lu YY, Liu XL, Huang Y, Liao Y, Xi T, Zhang YN, Zhang LL, Shu SN, Fang F (2019b) Short-lived AIM2 inflammasome activation relates to chronic MCMV infection in BALB/c mice. Curr Med Sci 39:899-905.
Lünemann JD, Malhotra S, Shinohara ML, Montalban X, Comabella M (2021) Targeting inflammasomes to treat neurological diseases. Ann Neurol 90:177-188.
Ma YL, Zhang LX, Liu GL, Fan Y, Peng Y, Hou WG (2017) N-Myc downstream-regulated gene 2 (Ndrg2) is involved in ischemia-hypoxia-induced astrocyte apoptosis: a novel target for stroke therapy. Mol Neurobiol 54:3286-3299.
Malireddi RK, Ippagunta S, Lamkanfi M, Kanneganti TD (2010) Cutting edge: proteolytic inactivation of poly(ADP-ribose) polymerase 1 by the Nlrp3 and Nlrc4 inflammasomes. J Immunol 185:3127-3130.
Malireddi RKS, Kesavardhana S, Kanneganti TD (2019) ZBP1 and TAK1: master regulators of NLRP3 inflammasome/pyroptosis, apoptosis, and necroptosis (PAN-optosis). Front Cell Infect Microbiol 9:406.
Malireddi RKS, Kesavardhana S, Karki R, Kancharana B, Burton AR, Kanneganti TD (2020a) RIPK1 distinctly regulates yersinia-induced inflammatory cell death, PANoptosis. ImmunoHorizons 4:789-796.
Malireddi RKS, Gurung P, Mavuluri J, Dasari TK, Klco JM, Chi H, Kanneganti TD (2018) TAK1 restricts spontaneous NLRP3 activation and cell death to control myeloid proliferation. J Exp Med 215:1023-1034.
Malireddi RKS, Gurung P, Kesavardhana S, Samir P, Burton A, Mummareddy H, Vogel P, Pelletier S, Burgula S, Kanneganti TD (2020b) Innate immune priming in the absence of TAK1 drives RIPK1 kinase activity-independent pyroptosis, apoptosis, necroptosis, and inflammatory disease. J Exp Med 217:jem.20191644.
Martinon F, Burns K, Tschopp J (2002) The inflammasome: a molecular platform triggering activation of inflammatory caspases and processing of proIL-beta. Mol Cell 10:417-426.
McBride DW, Zhang JH (2017) Precision stroke animal models: the permanent MCAO model should be the primary model, not transient MCAO. Transl Stroke Res doi: 10.1007/s12975-017-0554-2.
McKenzie BA, Dixit VM, Power C (2020) Fiery cell death: pyroptosis in the central nervous system. Trends Neurosci 43:55-73.
Meng H, Wu G, Zhao X, Wang A, Li D, Tong Y, Jin T, Cao Y, Shan B, Hu S, Li Y, Pan L, Tian X, Wu P, Peng C, Yuan J, Li G, Tan L, Wang Z, Li Y (2021) Discovery of a cooperative mode of inhibiting RIPK1 kinase. Cell Discov 7:41.
Meschia JF, Brott T (2018) Ischaemic stroke. Eur J Neurol 25:35-40.
Mo Y, Sun YY, Liu KY (2020a) Autophagy and inflammation in ischemic stroke. Neural Regen Res 15:1388-1396.
Mo ZT, Liao YL, Zheng J, Li WN (2020b) Icariin protects neurons from endoplasmic reticulum stress-induced apoptosis after OGD/R injury via suppressing IRE1α-XBP1 signaling pathway. Life Sci 255:117847.
Naito MG, Xu D, Amin P, Lee J, Wang H, Li W, Kelliher M, Pasparakis M, Yuan J (2020) Sequential activation of necroptosis and apoptosis cooperates to mediate vascular and neural pathology in stroke. Proc Natl Acad Sci U S A 117:4959-4970.
Neubert M, Ridder DA, Bargiotas P, Akira S, Schwaninger M (2011) Acute inhibition of TAK1 protects against neuronal death in cerebral ischemia. Cell Death Differ 18:1521-1530.
Ni Y, Gu WW, Liu ZH, Zhu YM, Rong JG, Kent TA, Li M, Qiao SG, An JZ, Zhang HL (2018) RIP1K contributes to neuronal and astrocytic cell death in ischemic stroke via activating autophagic-lysosomal pathway. Neuroscience 371:60-74.
Nikoletopoulou V, Markaki M, Palikaras K, Tavernarakis N (2013) Crosstalk between apoptosis, necrosis and autophagy. Biochim Biophys Acta 1833:3448-3459.
Ogura Y, Sutterwala FS, Flavell RA (2006) The inflammasome: first line of the immune response to cell stress. Cell 126:659-662.
Pender MP, Rist MJ (2001) Apoptosis of inflammatory cells in immune control of the nervous system: role of glia. Glia 36:137-144.
Poh L, Kang SW, Baik SH, Ng GYQ, She DT, Balaganapathy P, Dheen ST, Magnus T, Gelderblom M, Sobey CG, Koo EH, Fann DY, Arumugam TV (2019) Evidence that NLRC4 inflammasome mediates apoptotic and pyroptotic microglial death following ischemic stroke. Brain Behav Immun 75:34-47.
Ren Q, Hu Z, Jiang Y, Tan X, Botchway BOA, Amin N, Lin G, Geng Y, Fang M (2019) SIRT1 protects against apoptosis by promoting autophagy in the oxygen glucose deprivation/reperfusion-induced injury. Front Neurol 10:1289.
Ridder DA, Schwaninger M (2013) TAK1 inhibition for treatment of cerebral ischemia. Exp Neurol 239:68-72.
Rizzo MT, Leaver HA (2010) Brain endothelial cell death: modes, signaling pathways, and relevance to neural development, homeostasis, and disease. Mol Neurobiol 42:52-63.
Ryan F, Khodagholi F, Dargahi L, Minai-Tehrani D, Ahmadiani A (2018) Temporal pattern and crosstalk of necroptosis markers with autophagy and apoptosis associated proteins in ischemic hippocampus. Neurotox Res 34:79-92.
Ryou MG, Mallet RT (2018) An in vitro oxygen-glucose deprivation model for studying ischemia-reperfusion injury of neuronal cells. Methods Mol Biol 1717:229-235.
Salvador E, Burek M, Förster CY (2018) An in vitro model of traumatic brain injury. Methods Mol Biol 1717:219-227.
Samir P, Malireddi RKS, Kanneganti TD (2020) The PANoptosome: a deadly protein complex driving pyroptosis, apoptosis, and necroptosis (PANoptosis). Front Cell Infect Microbiol 10:238.
She Y, Shao L, Zhang Y, Hao Y, Cai Y, Cheng Z, Deng C, Liu X (2019) Neuroprotective effect of glycosides in Buyang Huanwu Decoction on pyroptosis following cerebral ischemia-reperfusion injury in rats. J Ethnopharmacol 242:112051.
Shi K, Tian DC, Li ZG, Ducruet AF, Lawton MT, Shi FD (2019) Global brain inflammation in stroke. Lancet Neurol 18:1058-1066.
Song M, Xia W, Tao Z, Zhu B, Zhang W, Liu C, Chen S (2021) Self-assembled polymeric nanocarrier-mediated co-delivery of metformin and doxorubicin for melanoma therapy. Drug Deliv 28:594-606.
Stanzione R, Forte M, Cotugno M, Bianchi F, Marchitti S, Rubattu S (2020) Role of DAMPs and of leukocytes infiltration in ischemic stroke: insights from animal models and translation to the human disease. Cell Mol Neurobiol doi: 10.1007/s10571-020-00966-4.
Sun L, Wang H, Wang Z, He S, Chen S, Liao D, Wang L, Yan J, Liu W, Lei X, Wang X (2012) Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148:213-227.
Sun R, Peng M, Xu P, Huang F, Xie Y, Li J, Hong Y, Guo H, Liu Q, Zhu W (2020) Low-density lipoprotein receptor (LDLR) regulates NLRP3-mediated neuronal pyroptosis following cerebral ischemia/reperfusion injury. J Neuroinflammation 17:330.
Tang M, Li X, Liu P, Wang J, He F, Zhu X (2018) Bradykinin B2 receptors play a neuroprotective role in Hypoxia/reoxygenation injury related to pyroptosis pathway. Curr Neurovasc Res 15:138-144.
Thapa RJ, Nogusa S, Chen P, Maki JL, Lerro A, Andrake M, Rall GF, Degterev A, Balachandran S (2013) Interferon-induced RIP1/RIP3-mediated necrosis requires PKR and is licensed by FADD and caspases. Proc Natl Acad Sci U S A 110:E3109-3118.
Tuttolomondo A, Di Sciacca R, Di Raimondo D, Renda C, Pinto A, Licata G (2009) Inflammation as a therapeutic target in acute ischemic stroke treatment. Curr Top Med Chem 9:1240-1260.
Vieira M, Fernandes J, Carreto L, Anuncibay-Soto B, Santos M, Han J, Fernández-López A, Duarte CB, Carvalho AL, Santos AE (2014) Ischemic insults induce necroptotic cell death in hippocampal neurons through the up-regulation of endogenous RIP3. Neurobiol Dis 68:26-36.
Virani SS, Alonso A, Benjamin EJ, Bittencourt MS, Callaway CW, Carson AP, Chamberlain AM, Chang AR, Cheng S, Delling FN, Djousse L, Elkind MSV, Ferguson JF, Fornage M, Khan SS, Kissela BM, Knutson KL, Kwan TW, Lackland DT, Lewis TT, et al. (2020) Heart Disease and Stroke Statistics-2020 Update: A Report From the American Heart Association. Circulation 141:e139-e596.
Voet S, Srinivasan S, Lamkanfi M, van Loo G (2019) Inflammasomes in neuroinflammatory and neurodegenerative diseases. EMBO Mol Med 11:e10248.
Wallach D, Kovalenko A, Kang TB (2011) ‘Necrosome’-induced inflammation: must cells die for it? Trends Immunol 32:505-509.
Wang K, Sun Z, Ru J, Wang S, Huang L, Ruan L, Lin X, Jin K, Zhuge Q, Yang S (2020a) Ablation of GSDMD improves outcome of ischemic stroke through blocking canonical and non-canonical inflammasomes dependent pyroptosis in microglia. Front Neurol 11:577927.
Wang L, Wu D, Xu Z (2019) USP10 protects against cerebral ischemia injury by suppressing inflammation and apoptosis through the inhibition of TAK1 signaling. Biochem Biophys Res Commun 516:1272-1278.
Wang M, Wan H, Wang S, Liao L, Huang Y, Guo L, Liu F, Shang L, Huang J, Ji D, Xia X, Jiang B, Chen D, Xiong K (2020b) RSK3 mediates necroptosis by regulating phosphorylation of RIP3 in rat retinal ganglion cells. J Anat 237:29-47.
Wang N, Wu L, Cao Y, Wang Y, Zhang Y (2013) The protective activity of imperatorin in cultured neural cells exposed to hypoxia re-oxygenation injury via anti-apoptosis. Fitoterapia 90:38-43.
Wang Q, Yin XH, Liu Y, Zhang GY (2011) K252a suppresses neuronal cells apoptosis through inhibiting the translocation of Bax to mitochondria induced by the MLK3/JNK signaling after transient global brain ischemia in rat hippocampal CA1 subregion. J Recept Signal Transduct Res 31:307-313.
Wang Q, Wu J, Zeng Y, Chen K, Wang C, Yang S, Sun N, Chen H, Duan K, Zeng G (2020c) Pyroptosis: a pro-inflammatory type of cell death in cardiovascular disease. Clin Chim Acta 510:62-72.
Wang QS, Luo XY, Fu H, Luo Q, Wang MQ, Zou DY (2020d) MiR-139 protects against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced nerve injury through targeting c-Jun to inhibit NLRP3 inflammasome activation. J Stroke Cerebrovasc Dis 29:105037.
Wang W, Xie L, Zou X, Hu W, Tian X, Zhao G, Chen M (2021a) Pomelo peel oil suppresses TNF-α-induced necroptosis and cerebral ischaemia-reperfusion injury in a rat model of cardiac arrest. Pharm Biol 59:401-409.
Wang WY, Xie L, Zou XS, Li N, Yang YG, Wu ZJ, Tian XY, Zhao GY, Chen MH (2021b) Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest. Int Immunopharmacol 93:107377.
Wang Y, Guan X, Gao CL, Ruan W, Zhao S, Kai G, Li F, Pang T (2021c) Medioresinol as a novel PGC-1α activator prevents pyroptosis of endothelial cells in ischemic stroke through PPARα-GOT1 axis. Pharmacol Res 169:105640.
Wang Z, Guo LM, Wang Y, Zhou HK, Wang SC, Chen D, Huang JF, Xiong K (2018) Inhibition of HSP90α protects cultured neurons from oxygen-glucose deprivation induced necroptosis by decreasing RIP3 expression. J Cell Physiol 233:4864-4884.
Wu CX, Wang TF, Yu JQ (2015) Lycium barbarum polysaccharide pretreatment attenuates cerebral ischemic reperfusion injury by inhibiting apoptosis in mice. Zhong Yao Cai 38:1454-1459.
Wu F, Zhang R, Feng Q, Cheng H, Xue J, Chen J (2020a) (-)-Clausenamide alleviated ER stress and apoptosis induced by OGD/R in primary neuron cultures. Neurol Res 42:730-738.
Wu X, Lin L, Qin JJ, Wang L, Wang H, Zou Y, Zhu X, Hong Y, Zhang Y, Liu Y, Xin C, Xu S, Ye S, Zhang J, Xiong Z, Zhu L, Li H, Chen J, She ZG (2020b) CARD3 promotes cerebral ischemia-reperfusion injury via activation of TAK1. J Am Heart Assoc 9:e014920.
Xu P, Zhang X, Liu Q, Xie Y, Shi X, Chen J, Li Y, Guo H, Sun R, Hong Y, Liu X, Xu G (2019) Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke. Cell Death Dis 10:555.
Yan PJ, Hou LS, Li ME, Lu ZX, Zhan FY, Ran MD, Li JJ, Zhang L, Yang R, Zhou MK, Zhu CR (2020a) Associations between lesion locations and stroke recurrence in survivors of first-ever ischemic stroke: a prospective cohort study. Curr Med Sci 40:708-718.
Yan W, Wang Z, Lu S, Li J, Chen Q, Wang L, Chen S, Wang X, Xiong K, Yan J (2020b) Analysis of factors related to prognosis and death of fish bile poisoning in China: a retrospective study. Basic Clin Pharmacol Toxicol 127:419-428.
Yan WT, Lu S, Yang YD, Ning WY, Cai Y, Hu XM, Zhang Q, Xiong K (2021) Research trends, hot spots and prospects for necroptosis in the field of neuroscience. Neural Regen Res 16:1628-1637.
Yanamoto H, Nagata I, Niitsu Y, Xue JH, Zhang Z, Kikuchi H (2003) Evaluation of MCAO stroke models in normotensive rats: standardized neocortical infarction by the 3VO technique. Exp Neurol 182:261-274.
Yang M, Lv Y, Tian X, Lou J, An R, Zhang Q, Li M, Xu L, Dong Z (2017) Neuroprotective effect of β-caryophyllene on cerebral ischemia-reperfusion injury via regulation of necroptotic neuronal death and inflammation: in vivo and in vitro. Front Neurosci 11:583.
Yang Y, Gao H, Liu W, Liu X, Jiang X, Li X, Wu Q, Xu Z, Zhao Q (2021) Arctium lappa L. roots ameliorates cerebral ischemia through inhibiting neuronal apoptosis and suppressing AMPK/mTOR-mediated autophagy. Phytomedicine 85:153526.
Yu Y, Wu X, Pu J, Luo P, Ma W, Wang J, Wei J, Wang Y, Fei Z (2018) Lycium barbarum polysaccharide protects against oxygen glucose deprivation/reoxygenation-induced apoptosis and autophagic cell death via the PI3K/Akt/mTOR signaling pathway in primary cultured hippocampal neurons. Biochem Biophys Res Commun 495:1187-1194.
Yuan J, Yankner BA (2000) Apoptosis in the nervous system. Nature 407:802-809.
Yuan J, Amin P, Ofengeim D (2019) Necroptosis and RIPK1-mediated neuroinflammation in CNS diseases. Nat Rev Neurosci 20:19-33.
Yuan P, Liu Z, Liu M, Huang J, Li X, Zhou X (2013) Up-regulated tumor necrosis factor-associated factor 6 level is correlated with apoptosis in the rat cerebral ischemia and reperfusion. Neurol Sci 34:1133-1138.
Yuan Z, Yi-Yun S, Hai-Yan Y (2020) Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis. Biomed Pharmacother 128:110045.
Zeng Q, Zhou Y, Liang D, He H, Liu X, Zhu R, Zhang M, Luo X, Wang Y, Huang G (2020) Exosomes secreted from bone marrow mesenchymal stem cells attenuate oxygen-glucose deprivation/reoxygenation-induced pyroptosis in PC12 cells by promoting AMPK-dependent autophagic flux. Front Cell Neurosci 14:182.
Zeyen T, Noristani R, Habib S, Heinisch O, Slowik A, Huber M, Schulz JB, Reich A, Habib P (2020) Microglial-specific depletion of TAK1 is neuroprotective in the acute phase after ischemic stroke. J Mol Med (Berl) 98:833-847.
Zhang D, Qian J, Zhang P, Li H, Shen H, Li X, Chen G (2019a) Gasdermin D serves as a key executioner of pyroptosis in experimental cerebral ischemia and reperfusion model both in vivo and in vitro. J Neurosci Res 97:645-660.
Zhang JF, Shi LL, Zhang L, Zhao ZH, Liang F, Xu X, Zhao LY, Yang PB, Zhang JS, Tian YF (2016) MicroRNA-25 negatively regulates cerebral ischemia/reperfusion injury-induced cell apoptosis through Fas/FasL pathway. J Mol Neurosci 58:507-516.
Zhang P, Zhang Y, Zhang J, Wu Y, Jia J, Wu J, Hu Y (2013) Early exercise protects against cerebral ischemic injury through inhibiting neuron apoptosis in cortex in rats. Int J Mol Sci 14:6074-6089.
Zhang Y, Wang H, Li H, Nan L, Xu W, Lin Y, Chu K (2021) Gualou Guizhi granule protects against OGD/R-induced injury by inhibiting cell pyroptosis via the PI3K/Akt signaling pathway. Evid Based Complement Alternat Med 2021:6613572.
Zhang Y, Li M, Li X, Zhang H, Wang L, Wu X, Zhang H, Luo Y (2020) Catalytically inactive RIP1 and RIP3 deficiency protect against acute ischemic stroke by inhibiting necroptosis and neuroinflammation. Cell Death Dis 11:565.
Zhang YY, Liu WN, Li YQ, Zhang XJ, Yang J, Luo XJ, Peng J (2019b) Ligustroflavone reduces necroptosis in rat brain after ischemic stroke through targeting RIPK1/RIPK3/MLKL pathway. Naunyn Schmiedebergs Arch Pharmacol 392:1085-1095.
Zhao SC, Ma LS, Chu ZH, Xu H, Wu WQ, Liu F (2017) Regulation of microglial activation in stroke. Acta Pharmacol Sin 38:445-458.
Zheng M, Kanneganti TD (2020a) Newly identified function of caspase-6 in ZBP1-mediated innate immune responses, NLRP3 inflammasome activation, PANoptosis, and host defense. J Cell Immunol 2:341-347.
Zheng M, Kanneganti TD (2020b) The regulation of the ZBP1-NLRP3 inflammasome and its implications in pyroptosis, apoptosis, and necroptosis (PANoptosis). Immunol Rev 297:26-38.
Zheng M, Williams EP, Malireddi RKS, Karki R, Banoth B, Burton A, Webby R, Channappanavar R, Jonsson CB, Kanneganti TD (2020) Impaired NLRP3 inflammasome activation/pyroptosis leads to robust inflammatory cell death via caspase-8/RIPK3 during coronavirus infection. J Biol Chem 295:14040-14052.
Zhou L, Ao LY, Yan YY, Li WT, Ye AQ, Li CY, Shen WY, Liang BW, Xiong Z, Li YM (2019) JLX001 ameliorates ischemia/reperfusion injury by reducing neuronal apoptosis via down-regulating JNK signaling pathway. Neuroscience 418:189-204.
Zhu S, Zhang Z, Jia LQ, Zhan KX, Wang LJ, Song N, Liu Y, Cheng YY, Yang YJ, Guan L, Min DY, Yang GL (2019) Valproic acid attenuates global cerebral ischemia/reperfusion injury in gerbils via anti-pyroptosis pathways. Neurochem Int 124:141-151.
Zou X, Xie L, Wang W, Zhao G, Tian X, Chen M (2020) FK866 alleviates cerebral pyroptosis and inflammation mediated by Drp1 in a rat cardiopulmonary resuscitation model. Int Immunopharmacol 89:107032.
Zou XS, Xie L, Wang WY, Zhao GY, Tian XY, Chen MH (2021) Pomelo peel oil alleviates cerebral NLRP3 inflammasome activation in a cardiopulmonary resuscitation rat model. Exp Ther Med 21:233.
C-Editor: Zhao M; S-Editors: Yu J, Li CH; L-Editors: Yu J, Song LP; T-Editor: Jia Y
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7]