Figure 2: Core components of the Notch signaling pathway. Schematic of the Notch signaling pathway highlighting points of possible regulation during retinal regeneration. 1: Notch receptors are post-translationally modified by glycosylation and Furin-dependent S1 cleavage. 2: Notch receptors on the signal-receiving cell bind ligands on the signal-sending cell. 3: Transendocytosis pulls the ligand-NECD complex into the signal-sending cell. 4: S2 site is available for metalloprotease-dependent cleavage. 5: γ-Secretase-dependent S3 cleavage releases NICD from the membrane. 6: NICD translocates into the nucleus of the signal-receiving cell and forms a complex with CSL transcription factor RBP-Jκ, MAML, and co-activators. 7: Notch binding of the chromatin ultimately induces expression changes of NICD target genes. Co-A: Co-activator protein; Co-R: co-repressor protein; CSL: (CBF1/RBP-Jκ, Su(H), LAG-1); MAML: Mastermind-like; NECD: Notch extracellular domain; NICD: Notch intracellular domain; S1: cleavage site 1; S2: cleavage site 2; S3: cleavage site 3.