Figure 2: EA improves cerebral blood perfusion and S1-M1 coherence in mice within 24 hours of unilateral focal photothrombotic infarct in M1. (A–C) Blood perfusion was detected using laser speckle blood flow imaging. Blood perfusion of the entire brain (A), contralateral M1 (B), and S1 (C) after stroke was rescued by EA treatment. (D) Five days after CTB488 injection, CTB488-positive neurons in S1 (green) projected from M1. CTB-488 was injected into M1 of wild-type control mice. Blue: DAPI. A confocal microscope was used to detect CTB488-positive neurons. Scale bars: 50 μm. (E) Neuronal activity of contralateral S1 and M1 was simultaneously recorded to analyze coherence. The coherence relationship between S1 and M1 after stroke was enhanced by EA treatment. All experiments were repeated five times. Data are presented as means ± standard error of the mean (see [Table 1] for the number of mice used in [Figure 2]). Number of units = 96 in the sham, stroke, and stroke + EA groups, respectively. Units were categorized as neuronal clusters with different discharge frequency and waveforms. *P < 0.05, **P < 0.01 (one-way analysis of variance with post hoc Bonferroni correction). CTB: Cholera Toxin Subunit B; EA: electroacupuncture; M1: primary motor cortex; S1: primary somatosensory cortex.