Figure 4: Excitability in Layer 2/3 and Layer 5/6 neurons located in contralateral S1 was demonstrated using in vitro slice electrophysiological recording within 24 hours of unilateral focal photothrombotic infarct in M1. (A1–A4) The resting membrane potential (resting MP) (A1), input resistance (Rin) (A2), threshold to spike (A3), and number of spikes induced at 300 pA current injection (Spike #) (A4) was not significantly different among the sham, stroke, and stroke + EA groups in Layer 2/3. Number of neurons = 12, 11, and 9 in the sham, stroke, and stroke + EA groups, respectively. (B1–B4) In Layer 5/6, the resting membrane potential (B1), input resistance (B2), and threshold to spike (B3) were not affected by stroke or EA treatment. However, the number of spikes induced at 300 pA current injection was significantly higher in the stroke group than the sham group (P < 0.01) but significantly lower in the stroke + EA group than the stroke group (P < 0.05). Number of neurons = 13, 12, and 11 in the sham, stroke, and stroke + EA groups, respectively. All experiments were repeated at least five times. Data are presented as meana ± standard error of the mean. See [Table 1] for the number of mice used in [Figure 4]. *P < 0.05, **P < 0.01 (one-way analysis of variance with post hoc Bonferroni correction). EA: Electroacupuncture; L2/3: Layer 2/3; L5/6: Layer 5/6.