Figure 6: After S1 ablation, EA treatment at GV14 and GV20 does not enhance neuronal activity or blood perfusion of contralateral M1 and S1 within 24 hours of unilateral focal photothrombotic infarction in M1. (A) taCasp3 virus (including rAAV-hsyn-taCasp3-TEVp-p2A-EGFP-WRPEs and rAAV-hsyn-taCasp3-p2A-EGFP-WRPEs) were injected into contralateral S1. The density of NeuN+ neurons was measured within the infected area. The number of NeuN+ neurons was ablated significantly in the taCasp3-TEVp group compared with the taCasp3-EGFP group (P < 0.05). Green, neurons expressing the taCasp3-EGFP virus; Blue, DAPI. (B1) Samples showing raster and spectrum in spike continuous in M1 after S1 ablation. Time shown is 300 seconds. (B2) Spike frequency in Layer 5/6 in M1 was not significantly different between the groups after S1 ablation. Number of units = 57, 51, and 83 in the sham, stroke, and stroke + EA groups. (C) EA treatment lost its efficacy with respect to blood perfusion of the entire brain after S1 ablation. (D) EA treatment lost its efficacy with respect to blood perfusion of the contralateral M1 after S1 ablation. (E) EA treatment lost its efficacy with respect to blood perfusion of the contralateral S1 after S1 ablation. All experiments were repeated at least five times. Data are presented as means ± standard error of the mean. *P < 0.05, **P < 0.01 (unpaired t-test in A, and one-way analysis of variance with post hoc Bonferroni correction in B–E). CTB: Cholera Toxin Subunit B; EA: electroacupuncture.