Figure 1: Establishment of zebrafish SCI model and evaluation of motor function after SCI. (A) Experimental design scheme. Zebrafish SCI surgery was performed on day 0 in a dark environment. On day 3, the zebrafish were returned to normal feeding and light/dark cycle conditions, and abnormal fish in the shape of “C” or “S” were removed. Zebrafish motor ability was measured on days 3, 7, and 14 after SCI. The RNA-sequencing datasets relating to samples from zebrafish on day 14 after SCI were downloaded for bioinformatics analysis, and the RT-qPCR validation was performed. (B) Zebrafish model of SCI. A longitudinal incision was made with scissors on the side of the zebrafish to expose the vertebral column. We cut the spinal cord about 4 mm caudal to the brainstem/spinal cord transitional junction between two vertebrae. A sham control lesion was performed by making the incision to expose the spinal cord. (C, D) Swim tracking. The movement of zebrafish was tracked and recorded with a camera for 5 minutes in a quiet environment. The total swimming distance of zebrafish in the tank in 5 minutes was calculated using the ImageJ’s Animal Tracker program (Schneider et al., 2012). ***P < 0.001 (Student’s t-test). Data were expressed as the mean ± SEM (n = 10). RT-qPCR: Real-time quantitative polymerase chain reaction; SCI: spinal cord injury.