Figure 5: Relative expression of target genes NONRATT020851.2 and NONRATT007410.2 at 1, 4, and 7 days following spinal cord injury. (A) Gene expression patterns of NONRATT020851.2 from sequencing data. (B–D) Gene expression of NONRATT020851.2 was determined by qRT-PCR using Actb (B), Tbp (C), and Ankrd27+Ubc (D) as internal controls. (E) Gene expression patterns of NONRATT007410.2 from sequencing data. (F–H) Gene expression of NONRATT007410.2 was determined by qRT-PCR using Actb (F), Tbp (G), and Ankrd27+Ubc (H) as internal controls. Actb is an unstable internal control, and Tbp and Ankrd27+Ubc are stable internal controls. Data are expressed as mean ± SD (n = 3 rats/group). *P < 0.05, vs. control group (Con) using one-way analysis of variance followed by the Holm-Šídák multiple comparisons test. Actb: Beta-Actin; Ankrd27: Ankyrin repeat domain 27; Tbp: TATA box binding protein; Ubc: ubiquitin C.