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  Indian J Med Microbiol
 

Figure 5: Relative expression of target genes NONRATT020851.2 and NONRATT007410.2 at 1, 4, and 7 days following spinal cord injury. (A) Gene expression patterns of NONRATT020851.2 from sequencing data. (B–D) Gene expression of NONRATT020851.2 was determined by qRT-PCR using Actb (B), Tbp (C), and Ankrd27+Ubc (D) as internal controls. (E) Gene expression patterns of NONRATT007410.2 from sequencing data. (F–H) Gene expression of NONRATT007410.2 was determined by qRT-PCR using Actb (F), Tbp (G), and Ankrd27+Ubc (H) as internal controls. Actb is an unstable internal control, and Tbp and Ankrd27+Ubc are stable internal controls. Data are expressed as mean ± SD (n = 3 rats/group). *P < 0.05, vs. control group (Con) using one-way analysis of variance followed by the Holm-Šídák multiple comparisons test. Actb: Beta-Actin; Ankrd27: Ankyrin repeat domain 27; Tbp: TATA box binding protein; Ubc: ubiquitin C.

<b>Figure 5: Relative expression of target genes <i>NONRATT020851.2</i> and <i>NONRATT007410.2</i> at 1, 4, and 7 days following spinal cord injury.</b>
(A) Gene expression patterns of <i>NONRATT020851.2</i> from sequencing data. (B–D) Gene expression of <i>NONRATT020851.2</i> was determined by qRT-PCR using <i>Actb</i> (B), <i>Tbp</i> (C), and <i>Ankrd27+Ubc</i> (D) as internal controls. (E) Gene expression patterns of <i>NONRATT007410.2</i> from sequencing data. (F–H) Gene expression of <i>NONRATT007410.2</i> was determined by qRT-PCR using <i>Actb</i> (F), <i>Tbp</i> (G), and <i>Ankrd27+Ubc</i> (H) as internal controls. <i>Actb</i> is an unstable internal control, and <i>Tbp</i> and <i>Ankrd27+Ubc</i> are stable internal controls. Data are expressed as mean ± SD (<i>n</i> = 3 rats/group). *<i>P</i> < 0.05, <i>vs</i>. control group (Con) using one-way analysis of variance followed by the Holm-Šídák multiple comparisons test. Actb: Beta-Actin; Ankrd27: Ankyrin repeat domain 27; Tbp: TATA box binding protein; Ubc: ubiquitin C.