Figure 2: Human induced pluripotent stem cells-derived iXCellsTM motor neurons. (A) More than 85% of the iXCellsTM motor neurons derived from human induced pluripotent stem cells (iPSCs) expressed an early motor neuronal marker, HB9. The cells were co-labeled with the neuronal marker Tuj1 and the nuclear marker 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI). (B) A confocal image of iXCellsTM motor neurons co-cultured with C2C12 mouse myotubes for 6 days. Neuromuscular junctions were stained with α-bungarotoxin (BTX). Motor neurons were stained for neurofilament and MAP2. Scale bars: 150 μm in A, 50 μm in B. Unpublished data.